Table of Contents

2017 Month : April Volume : 3 Issue : 1 Page : 1-3

DETECTION AND DISTRIBUTION PATTERN OF PREVALENT GENOTYPES OF HEPATITIS C VIRUS IN A TERTIARY CARE HOSPITAL IN NORTH INDIA.

Saumya Pallavi Lall1, Aroma Oberoi2, Gurvinder Singh Chopra3

Corresponding Author:
Dr. Saumya Pallavi Lall,
Department of Microbiology,
Christian Medical College and Hospital,
Ludhiana, Punjab, India.
E-mail: dr.saumyalall@gmail.com

ABSTRACT

BACKGROUND

Hepatitis C virus (HCV) has emerged as a leading cause of chronic hepatitis and hepatocellular carcinoma. Thus this study was undertaken for detection and to observe genotypic distribution of the virus in this geographical region.

The aim of the study is to quantitatively detect HCV-RNA in Hepatitis C patients and to determine the distribution pattern of its genotypes by real time polymerase chain reaction.

MATERIALS AND METHODS

This study was conducted in a tertiary care hospital in north India from January 1, 2013 to June 30, 2015. Quantification of HCV-RNA was done by real time PCR and in HCV-RNA positive samples, genotyping for HCV was conducted.

 

RESULTS

A total of 465 patients were recruited in the study, of which 217 were anti-HCV positive and 420 were HCV-RNA positive. These positive samples were further subjected to genotype determination using real time PCR. 163 samples were subjected to genotyping, where genotype 3 was the commonest as seen in 102 patients, followed by genotype 1 seen in 25 patients and genotype 4 in 8 patients, and in 27 patients genotype was undetected.

CONCLUSION

Without reliable assays for antigenemia and the inability of antibody tests to define viraemia in all cases, the detection of viral nucleic acid is necessary for diagnosis of active HCV infection. Genotype is clinically important in determining potential response and duration to interferon-based therapy. Genotype 1 and 4 are less responsive to interferon based treatment than are the other genotypes (2, 3, 5 and 6). Duration of standard interferon based therapy for genotypes 1 and 4 is 48 weeks, whereas treatment for genotypes 2 and 3 is completed in 24 weeks.

Keywords

Hepatitis C Virus, Anti-HCV, HCV-RNA, HCV Genotype.

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